General Cell Based Assays
Eurofins Discoverx PathHunter eXpress PTHR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0315E2CP0L)
The PathHunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR1 and EA-tagged β-Arrestin-2. Activation of PTHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IP-10 ASY
The Meso Scale Discovery U-PLEX Mouse IP-10 Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of mouse interferon gamma-induced protein 10 (IP-10) in serum plasma and cell culture supernatants It utilizes electrochemiluminescent detection technology on patterned arrays for high sensitivity and broad dynamic range The assay requires 50 L or less sample volume and can be read on the MESO QuickPlex SQ 120 instrument The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and controls needed to perform the assay
Cayman Chemical SphngosIn KInase 2humn25ug
Source: Active recombinant N-terminal His-tagged protein expressed in insect cells • Amino acids: 2-654 (full length) • MW: 72.9 kDa
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 100. (93-0563R20A)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
![Genetex Inc Kallikrein 7 Monoclonal Antibody [MSVA-707M] HistoMAX™ (GTX04475) GeneTex - IHC-Optimized Antibody.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC3667782.jpg-250.jpg)
Genetex Inc Kallikrein 7 Monoclonal Antibody [MSVA-707M] HistoMAX™ (GTX04475) GeneTex - IHC-Optimized Antibody.
The Kallikrein 7 antibody [MSVA-707M] HistoMAX™ (GTX04475) is a Mouse Monoclonal antibody in the GeneTex HistoMAX portfolio. The antibody is extensively evaluated for IHC-P using formalin-fixed human normal tissues (more than 70 different sections) and cancer tissue arrays to guarantee specificity and best-in-class performance. Information regarding both manual and autostainer staining protocols are available.Figure Legend: IHC-P analysis of human skin tissue using GTX04475 Kallikrein 7 antibody [MSVA-707M] HistoMAX. A strong kallikrein 7 staining in the stratum granulosum and the keratinizing superficial layer of the skin.
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0235E3CP0L)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences Catalase fluorometric detection kit (500tests)
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided. Sensitivity: (range 0- 4 U/ml). UniProt ID: P00432. Applications: Fluorescent detection, HTS . Species reactivity: Species independent . Content: Reaction buffer concentrate, Detection Reagent, Horseradish peroxidase concentrate, Hydrogen peroxide, Catalase enzyme.
Enzo Life Sciences Matrix Metalloproteinase-7 (MMP-7) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-7 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-7, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH)3, and a detailed instruction booklet. Alternative name: Matrilysin, Pump. Applications: Fluorescent detection, HTS Activity assay.
AURORA BIOLABS LLC Cov-19 NP Rabbit Binding Assay, 384 rxn
SARS-CoV-2 Nucleocapsid protein (NP) is one of the core components of SARS-CoV-2 virus. It forms a complex with viral genomic RNA in a helical symmetrical structure and plays a key role in the process of virus replication and assembly. Since NP is abundantly expressed during infection, it can be used as an important diagnostic marker for COVID-19 and also can be used as a potential drug target or developing vaccines. Terbium-labeled anti-Tag1 antibody serves as fluorescence donor, that binds to the His-Tagged NP. If a test rabbit antibody binds to NP, fluorescence- labeled anti-rabbit antibody (fluorescence acceptor) will be brought in close proximity with the fluorescence donor. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled acceptor, which consequently fluoresces at 665 nm (figure below). Thus, the test antibody binding to NP can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Eurofins Discoverx cAMP Hunter eXpress FFAR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0136E2CP2L)
The cAMP Hunter FFAR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. FFAs) based activation of FFAR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FFAR3, a naturally Gi/Go coupled GPCR. Upon activation of FFAR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IGF1R Functional Assay, 2-Plate Kit. (93-0505E1CP17M)
The PathHunter eXpress IGF1R Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated IGF1R dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged IGF1R and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BMPR1A/BMPR2 Dimerization Assay, 2pl Kit. (93-1006E3CP0M)
The PathHunter BMPR1A/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-4) induced dimerization of BMPR1A/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses BMPR2 target protein fused to PK alongside BMPR1A target protein fused to EA. Upon ligand-induced dimerization of BMPR1A/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human BMP-4 ASY
The R-PLEX Human BMP-4 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures the concentration of human Bone Morphogenetic Protein 4 (BMP-4) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL
Abcam Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Human MCP-3 Assay, Single-Analyte, 96-well plate, LLOD 0.79 pg/mL, Dynamic Range 0.79 - 5000 pg/mL, Uses human serum, plasma, cell culture supernatant
The U-PLEX Human MCP-3 Assay from MESO SCALE DISCOVERY offers a robust solution for the quantitative measurement of human Monocyte Chemoattractant Protein 3 (MCP-3/CCL7). This assay is designed for research applications in immunology and inflammation, providing sensitive detection across a wide dynamic range of human biological samples.
- High quality and ease of use with validated components
- Enables precise quantification of human MCP-3, crucial for inflammatory response
- Validated for diverse human sample types, including serum, plasma, and cell culture supernatant
- Features a low limit of detection at 0.79 pg/mL
- Wide dynamic range of 0.79 - 5,000 pg/mL for various concentrations
- Single-analyte format optimized for dedicated MCP-3 measurement